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Department of Biomedical Engineering
GBE 403 | Course Introduction and Application Information
| Course Name |
Cell Culture Techniques
|
|
Code
|
Semester
|
Theory
(hour/week) |
Application/Lab
(hour/week) |
Local Credits
|
ECTS
|
|
GBE 403
|
SPRING
|
2
|
2
|
3
|
6
|
| Prerequisites | None | |||||
| Course Language | English | |||||
| Course Type | ELECTIVE_COURSE | |||||
| Course Level | First Cycle | |||||
| Mode of Delivery | Face to Face | |||||
| Teaching Methods and Techniques of the Course |
Discussion Group Work Q&A Lecture / Presentation |
|||||
| National Occupational Classification Code | - | |||||
| Course Coordinator |
|
|||||
| Course Lecturer(s) |
|
|||||
| Assistant(s) | - | |||||
| Course Objectives | The aim of this course is to use cell culture techniques, to be able to work under sterile conditions in cell culture | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Learning Outcomes |
The students who succeeded in this course;
|
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| Course Description | Cell culture techniques, including sterile techniques, primary cell culture, established cell lines, stem cell and tumor cell culture, as well as three-dimensional (3D) cell culture systems, will be introduced. | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| Related Sustainable Development Goals |
-
|
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|
|
Core Courses |
|
| Major Area Courses |
X
|
|
| Supportive Courses |
|
|
| Media and Managment Skills Courses |
|
|
| Transferable Skill Courses |
|
WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES
| Week | Subjects | Required Materials | Learning Outcome |
| 1 | Biology of Cultured Cells | Culture of Animal Cells-Chapter 2 | LO1 |
| 2 | Laboratory Design, Layout, Equipment and Materials | Culture of Animal Cells-Chapter 3 and 4 | LO1 |
| 3 | Aseptic Technique, Safety, Bioethics and Validation | Culture of Animal Cells-Chapter 5 and 6 | LO2 |
| 4 | Culture Vessels and Substrates and Defined and Serum-Free Media | Culture of Animal Cells-Chapter 7, 8 and 9 | LO1 |
| 5 | Preparation and Sterilization | Culture of Animal Cells-Chapter 10 | LO2 |
| 6 | Primary Culture | Culture of Animal Cells-Chapter 11 | LO4 |
| 7 | 1.Midterm | - | |
| 8 | Subculture and Cell Lines | Culture of Animal Cells-Chapter 12 | LO1 |
| 9 | Cloning and Selection | Culture of Animal Cells-Chapter 13 | LO3 |
| 10 | Cell Separation and Characterization | Culture of Animal Cells-Chapter 14 and 15 | LO4 |
| 11 | Differentiation, Transformation and Immortalization | Culture of Animal Cells-Chapter 16 and 17 | LO4 |
| 12 | 2.Midterm | - | |
| 13 | Contamination and Cryopreservation | Culture of Animal Cells-Chapter 18 and 19 | LO2 |
| 14 | Stem Cells, Germ Cells and Amniocytes | Culture of Animal Cells-Chapter 23 | LO5 |
| 15 | Culture of Tumor Cells and Three-Dimensional Culture | Culture of Animal Cells-Chapter 24 and 25 | LO5 |
| 16 | Final exam | - |
| Course Notes/Textbooks | Culture of Animal Cells. A Manual of Basic Technique and Specialized Applications 6th ed. By R. Ian Freshney. John Wiley&Sons (2010). |
| Suggested Readings/Materials | - |
EVALUATION SYSTEM
| Semester Activities | Number | Weighting | LO1 | LO2 | LO3 | LO4 | LO5 |
| Quizzes / Studio Critiques | 2 | 20 | X | X | |||
| Presentation / Jury | 1 | 10 | X | X | X | ||
| Project | 1 | 10 | X | X | |||
| Midterm | 1 | 30 | X | X | X | X | X |
| Final Exam | 1 | 30 | X | X | X | ||
| Total | 6 | 100 |
ECTS / WORKLOAD TABLE
| Semester Activities | Number | Duration (Hours) | Workload |
|---|---|---|---|
| Participation | - | - | - |
| Theoretical Course Hours | 16 | 2 | 32 |
| Laboratory / Application Hours | 16 | 2 | 32 |
| Study Hours Out of Class | 14 | 1 | 14 |
| Field Work | - | - | - |
| Quizzes / Studio Critiques | 2 | 15 | 30 |
| Portfolio | - | - | - |
| Homework / Assignments | - | - | - |
| Presentation / Jury | 1 | 20 | 20 |
| Project | 1 | 20 | 20 |
| Seminar / Workshop | - | - | - |
| Oral Exams | - | - | - |
| Midterms | 1 | 16 | 16 |
| Final Exam | 1 | 16 | 16 |
| Total | 180 |
COURSE LEARNING OUTCOMES AND PROGRAM QUALIFICATIONS RELATIONSHIP
| # | PC Sub | Program Competencies/Outcomes | * Contribution Level | ||||
| 1 | 2 | 3 | 4 | 5 | |||
| No program competency data found. | |||||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest
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