Department of Biomedical Engineering

GBE 340 | Course Introduction and Application Information

Course Name

Cell Death Mechanisms

Code	Semester	Theory (hour/week)	Application/Lab (hour/week)	Local Credits	ECTS
GBE 340	SPRING	3	0	3	5

Prerequisites	None
Course Language	English
Course Type	ELECTIVE_COURSE
Course Level	First Cycle
Mode of Delivery	Face to Face
Teaching Methods and Techniques of the Course	Discussion Group Work Q&A Lecture / Presentation
National Occupational Classification Code	-
Course Coordinator	Prof. Dr. Zeynep Fırtına Karagonlar
Course Lecturer(s)	Dr. Öğr. Üyesi Gizem Ayna Duran
Assistant(s)	-

Course Objectives

This course will focus on understanding the basics of conventional as well as unconventional cell death mechanisms and their roles in development, physiology and pathology. It will cover a broad range of topics in cell death field including apoptosis, autophagy, necrosis, necroptosis, lysosome-dependent cell death, entosis, anoikis, ferroptosis, pyroptosis, anastasis, mitotic death and immunogenic cell death.

Learning Outcomes

The students who succeeded in this course;

Name	Description	PC Sub	* Contribution Level
Name	Description	PC Sub	1	2	3	4	5
LO1	Explain the basic principles of cell death mechanisms and apply these principles to solve current problems in the field				X
LO2	Explain the methods used to analyse cell death mechanisms				X
LO3	Model cell death mechanisms, and will be able to report this model individually and present it orally				X
LO4	Explain the role of cell death mechanisms in embryonic development and how dysregulation of these mechanisms can lead to pathological conditions such as cancer and neurodegenerative diseases				X

Course Description

This course covers the description of molecular mechanisms of cell death including apoptosis, autophagy, necrosis, necroptosis, lysosome-dependent cell death, entosis, anoikis, ferroptosis, pyroptosis, anastasis, mitotic death and immunogenic cell death.

Related Sustainable Development Goals

Course Category	Core Courses
	Major Area Courses	X
	Supportive Courses
	Media and Managment Skills Courses
	Transferable Skill Courses

WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES

Week	Subjects	Required Materials	Learning Outcome
1	Introduction to cell death modalities	Alberts et.al. 6th Edition. Chapter 18.	LO1
2	Non-programmed cell death: Necrosis	Alberts et.al. 6th Edition. Chapter 18.	LO1
3	Programmed apoptotic cell death: Apoptotic Cell Death and Anoikis	Galluzzi, Lorenzo et al. “Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018” Cell death and differentiation vol. 25,3 (2018): 486-541.	LO1
4	Intrinsic and Extrinsic Apoptotic Cell Death	Galluzzi, Lorenzo et al. “Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018” Cell death and differentiation vol. 25,3 (2018): 486-541.	LO2
5	Apoptotic Cell Death Detection Methods	Hugh J. M. Brady. Apoptosis Methods and Protocols. Humana Press 2004. https://doi.org/10.1385/1592598129	LO2
6	Programmed non-apoptotic cell death: Vacuole based	Galluzzi, L et al. “Molecular definitions of cell death subroutines: recommendations of the Nomenclature Committee on Cell Death 2012” Cell death and differentiation vol. 19,1 (2011): 107-20.	LO2
7	Programmed non-apoptotic cell death: Mitochondria and iron based	Kroemer, G et al. “Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009” Cell death and differentiation vol. 16,1 (2008): 3-11.	LO2
8	Midterm		-
9	Programmed non-apoptotic cell death: Vacuole based: Immunogenic cell death and others	Galluzzi, Lorenzo et al. “Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018” Cell death and differentiation vol. 25,3 (2018): 486-541.	LO3
10	Programmed cell death in prokaryotes	Galluzzi, Lorenzo et al. “Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018” Cell death and differentiation vol. 25,3 (2018): 486-541.	LO3
11	Clinical aspects of cell death	Douglas R Green. Means to an End: Apoptosis and Other Cell Death Mechanisms. Cold Spring Harbor Laboratory Press. 2017. Galluzzi, Lorenzo et al. “Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018” Cell death and differentiation vol. 25,3 (2018): 486-541.	LO4
12	Non-lethal processes such as anastasis, mitotic destruction and cellular senescence	Hochman A. Programmed cell death in prokaryotes. Crit Rev Microbiol. 1997;23(3):207-14.	LO4
13	Cell-in-cell death (entosis, cannibalism and imperitosis)	Galluzzi L, Buqué A, Kepp O, Zitvogel L, Kroemer G. Immunogenic cell death in cancer and infectious disease. Nat Rev Immunol. 2017 Feb;17(2):97-111. doi: 10.1038/nri.2016.107. Epub 2016 Oct 17.	LO4
14	Review of the semester		-
15	Review of the semester		-
16	Final		-

Course Notes/Textbooks	Bruce Alberts- Alexander- Johnson Julian -Lewis David -Morgan- Martin Raff- Keith Roberts- Peter Walter. Molecular Biology of the Cell. Sixth Edition. Chapter 18 – Cell Death. Garland Science 2014: New York and Abingdon UK. Douglas R Green. Means to an End: Apoptosis and Other Cell Death Mechanisms. Cold Spring Harbor Laboratory Press. 2017.
Suggested Readings/Materials	-

EVALUATION SYSTEM

Semester Activities	Number	Weighting	LO1	LO2	LO3	LO4
Homework / Assignments	1	25			X	X
Presentation / Jury	1	25		X	X
Midterm	1	20	X	X
Final Exam	1	30	X	X		X
Total	4	100

ECTS / WORKLOAD TABLE

Semester Activities	Number	Duration (Hours)	Workload
Participation	-	-	-
Theoretical Course Hours	16	3	48
Laboratory / Application Hours	-	-	-
Study Hours Out of Class	16	2	32
Field Work	-	-	-
Quizzes / Studio Critiques	-	-	-
Portfolio	-	-	-
Homework / Assignments	1	10	10
Presentation / Jury	1	20	20
Project	-	-	-
Seminar / Workshop	-	-	-
Oral Exams	-	-	-
Midterms	1	20	20
Final Exam	1	20	20
		Total	150