Department of Biomedical Engineering

BME 420 | Course Introduction and Application Information

Course Name

PCR-based Molecular Diagnosis

Code	Semester	Theory (hour/week)	Application/Lab (hour/week)	Local Credits	ECTS
BME 420	SPRING	2	2	3	6

Prerequisites	None
Course Language	English
Course Type	ELECTIVE_COURSE
Course Level	First Cycle
Mode of Delivery	Face-To-Face
Teaching Methods and Techniques of the Course	Presentation/Discussion Laboratory Question&Answer Problem solving
National Occupational Classification Code	-
Course Coordinator	Doç. Dr. Osman Doluca
Course Lecturer(s)	Doç. Dr. Osman Doluca
Assistant(s)	Araş. Gör. Hüseyin Saygın Portakal

Course Objectives

The objective of this course to introduce the basics of polymerase chain reaction. Throughout the course students learn how to prepare samples and set up PCR instruments and evaluation of the results of PCR technique in diagnosis of genetical or communicable diseases.

Learning Outcomes

The students who succeeded in this course;

Name	Description	PC Sub	* Contribution Level
Name	Description	PC Sub	1	2	3	4	5
LO1	Will be able to describe the principles of Polymerase Chain Reaction (PCR)			X
LO2	Will be able to prepare components of PCR			X
LO3	Will be able to design primer and probe for PCR					X
LO4	Will be able to write PCR protocol				X
LO5	Will be able to evaluate the results obtained from the PCR device.					X

Course Description

The course covers the definition and history of polymerase chain reaction (PCR) method and how this method evolved through the years. The course covers principles of modern PCR methods and basic application of a PCR protocol starting from sample preparation to evaluation of the results obtained from PCR instruments. The course covers the introduction to common genetic and communicable diseases and PCR applications for their diagnosis in modern medicine.

Related Sustainable Development Goals

Course Category	Core Courses
	Major Area Courses	X
	Supportive Courses
	Media and Managment Skills Courses
	Transferable Skill Courses

WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES

Week	Subjects	Required Materials	Learning Outcome
1	Introduction to Molecular Diagnosis	Lecture Notes	LO1
2	Principles of Polymerase Chain Reaction (PCR)	Chapter 2, Viljoen, G. J., Nel, L. H., & Crowther, J. R. (2005). Molecular Diagnostic PCR handbook. Springer Science & Business Media. ISBN 978-1402034046	LO1
3	Reverse Transcriptase PCR	Chapter 5, Viljoen, G. J., Nel, L. H., & Crowther, J. R. (2005). Molecular Diagnostic PCR handbook. Springer Science & Business Media. ISBN 978-1402034046	LO1
4	Quantitative PCR	Chapter 5, Viljoen, G. J., Nel, L. H., & Crowther, J. R. (2005). Molecular Diagnostic PCR handbook. Springer Science & Business Media. ISBN 978-1402034046	LO1
5	Biological Samples and Isolation Methods	Lecture notes	LO2
6	PCR optimization	Lecture notes	LO2
7	Template design	Chapter 3, Viljoen, G. J., Nel, L. H., & Crowther, J. R. (2005). Molecular Diagnostic PCR handbook. Springer Science & Business Media. ISBN 978-1402034046	LO3
8	Homework		-
9	Primer design	Chapter 3, Viljoen, G. J., Nel, L. H., & Crowther, J. R. (2005). Molecular Diagnostic PCR handbook. Springer Science & Business Media. ISBN 978-1402034046	LO3
10	Prob design	Chapter 3, Viljoen, G. J., Nel, L. H., & Crowther, J. R. (2005). Molecular Diagnostic PCR handbook. Springer Science & Business Media. ISBN 978-1402034046	LO2
11	Setting Up PCR Protocol	Chapter 3, Viljoen, G. J., Nel, L. H., & Crowther, J. R. (2005). Molecular Diagnostic PCR handbook. Springer Science & Business Media. ISBN 978-1402034046	LO4
12	Post-PCR Melting Analysis	Lecture notes	LO4
13	Normalization Methods and Reporting	Chapter 3, Viljoen, G. J., Nel, L. H., & Crowther, J. R. (2005). Molecular Diagnostic PCR handbook. Springer Science & Business Media. ISBN 978-1402034046	LO5
14	PCR-based diagnosis of Genetic and Communicable Diseases	Chapter 7, Viljoen, G. J., Nel, L. H., & Crowther, J. R. (2005). Molecular Diagnostic PCR handbook. Springer Science & Business Media. ISBN 978-1402034046	LO5
15	Review and Lab Design for PCR		-
16	Final Exam		-

Course Notes/Textbooks	Viljoen G. J. Nel L. H. & Crowther J. R. (Eds.). (2005). Molecular diagnostic PCR handbook. Springer science & business media. ISBN 978-1402034046
Suggested Readings/Materials	-

EVALUATION SYSTEM

Semester Activities	Number	Weighting	LO1	LO2	LO3	LO4	LO5
Laboratory / Application	1	30	X	X	X	X	X
Homework / Assignments	1	30			X		X
Final Exam	1	40	X	X	X	X	X
Total	3	100

ECTS / WORKLOAD TABLE

Semester Activities	Number	Duration (Hours)	Workload
Participation	-	-	-
Theoretical Course Hours	16	2	32
Laboratory / Application Hours	16	2	32
Study Hours Out of Class	14	3	42
Field Work	-	-	-
Quizzes / Studio Critiques	-	-	-
Portfolio	-	-	-
Homework / Assignments	2	16	32
Presentation / Jury	-	-	-
Project	-	-	-
Seminar / Workshop	-	-	-
Oral Exams	-	-	-
Midterms	-	-	-
Final Exam	1	42	42
		Total	180